Journal
ACS NANO
Volume 3, Issue 7, Pages 2007-2015Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn900469q
Keywords
nanoparticle; nanoshell; TAT; laser; gold; silencing; RNA
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Funding
- Institute for Collaborative Biotechnologies (ICB) [DAAD19-03-D-0004]
- U.S. Army Research Office
- NIH [HL080718]
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The temporal and spatial control over the delivery of materials such as siRNA into cells remains a significant technical challenge. We demonstrate the pulsed near-infrared (NIR) laser-dependent release of siRNA from coated 40 nm gold nanoshells. Tat-lipid coating mediates the cellular uptake of the nanomaterial at picomolar concentration, while spatiotemporal silencing of a reporter gene (green fluorescence protein) was studied using photomasking. The NIR laser-induced release of siRNA from the nanoshells is found to be power-and time-dependent, through surface-linker bond cleavage, while the escape of the siRNA from endosomes occurs above a critical pulse energy attributed to local heating and cavitation. NIR laser-controlled drug release from functional nanomaterials should facilitate more sophisticated developmental biology and therapeutic studies.
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