Journal
ACS NANO
Volume 4, Issue 1, Pages 439-451Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn901322d
Keywords
mesoporous silica thin film; low molecular weight proteome; nanotexture; chemical modification; mass spectrometry; early diagnostics
Categories
Funding
- NSF [0618242]
- State of Texas's Emerging Technology Fund
- NIH [R21/R33CA122864, R01CA128797]
- NASA [NNJ06HE06A]
- DoD [W81XWH-08-BCRP-INNOV]
- NATIONAL CANCER INSTITUTE [R21CA122864, R33CA122864, U54CA151668, R01CA128797] Funding Source: NIH RePORTER
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We present a fast, efficient, and reliable system based on mesoporous silica chips to specifically fractionate and enrich the low molecular weight proteome. Mesoporous silica thin films with tunable features at the nanoscale were fabricated using the triblock copolymer template pathway. Using different templates and concentrations in the precursor solution, various pore size distributions, pore structures, and connectivity were obtained and applied for selective recovery of low mass proteins. In combination with mass spectrometry and statistic analysis, we demonstrated the correlation between the nanophase characteristics of the mesoporous silica thin films and the specificity, and. efficacy, of low mass proteome harvesting. In addition, to overcome the limitations of the prefunctionalization method in polymer selection, plasma ashing was used for the first time for the treatment of the mesoporous silica surface prior to chemical modification. Surface charge modifications by different functional groups resulted in a selective capture of the low molecular weight proteins from serum sample. In conclusion, our study demonstrates that the ability to tune the physicochemical properties of mesoporous silica surfaces, for a selective:enrichment of the low molecular weight proteome from complex biological fluids, has the potential to promote proteomic biomarker discovery.
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