4.8 Article

Synthetic Polymer Nanoparticles with Antibody-like Affinity for a Hydrophilic Peptide

Journal

ACS NANO
Volume 4, Issue 1, Pages 199-204

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/nn901256s

Keywords

selective peptide capture; synthetic nanoparticles; molecular imprinting; QCM; inverse microemulsion polymerization; plastic antibodies

Funding

  1. National Institutes of Health
  2. NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM080506] Funding Source: NIH RePORTER

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Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide-NP binding affinities (ca. 90-900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the nanoparticles. This method can be used for the preparation of nanosized synthetic polymers with antibody-like affinity for hydrophilic peptides and proteins (plastic antibodies).

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