Journal
IMMUNOLOGY LETTERS
Volume 167, Issue 2, Pages 131-140Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.imlet.2015.08.004
Keywords
B cells; Salmonella; CD8 T cells; Cross-presentation; PD-L1
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Funding
- Conacyt-SEP [132310]
- Conacyt-CVU [228852]
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Salmonella persists for a long time in B cells; however, the mechanism(s) through which infected B cells avoid effector CD8 T cell responses has not been characterized. In this study, we show that Salmonella infects and survives within all B1 and B2 cell subpopulations. B cells are infected with a Salmonella typhimurium strain expressing an ovalbumin (OVA) peptide (SIINFEKL) to evaluate whether B cells process and present Salmonella antigens in the context of MHC-I molecules. Our data showed that OVA peptides are presented by MHC class I K-b-restricted molecules and the presented antigen is generated through proteasomal degradation and vacuolar processing. In addition, Salmonella-infected B cells express costimulatory molecules such as CD40, CD80, and CD86 as well as inhibitory molecules such as PD-L1. Thus, the cross-presentation of Salmonella antigens and the expression of activation molecules suggest that infected B cells are able to prime and activate specific CD8(+)T cells. However, the Salmonella infection-stimulated expression of PD-L1 suggests that the PD-1/PD-L1 pathway may be involved in turning off the cytotoxic effector response during Salmonella persistent infection, thereby allowing B cells to become a reservoir for the bacteria. (C) 2015 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.
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