Journal
ACS NANO
Volume 2, Issue 8, Pages 1653-1660Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn800136e
Keywords
biosensor; DNA/PNA hybridization; surface characterization; XPS; X-ray reflectivity; organophosphonate monolayers; heterobifunctional linker; silicon nanowire device
Categories
Funding
- DFG
- Nanosystems Initiative Munich
- Fujitsu Laboratories of Europe
- BMBF [03N8713, 03X5513]
- National Science Foundation [CHE-0612572]
Ask authors/readers for more resources
We investigated hydroxyalkylphosphonate monolayers as a novel platform for the biofunctionalization of silicon-based field effect sensor devices. This included a detailed study of the thin film properties of organophosphonate films on Si substrates using several surface analysis techniques, including AFM, ellipsometry, contact angle, X-ray photoelectron spectroscopy (XPS), X-ray reflectivity, and current-voltage characteristics in electrolyte solution. Our results indicate the formation of a dense monolayer on the native silicon oxide that has excellent passivation properties. The monolayer was biofunctionalized with 12 mer peptide nucleic acid (PNA) receptor molecules in a two-step procedure using the heterobifunctional linker, 3-maleimidopropionic-acid-N-hydroxysuccinimidester. Successful surface modification with the probe PNA was verified by XPS and contact angle measurements, and hybridization with DNA was determined by fluorescence measurements. Finally, the PNA functionalization protocol was translated to 2 gm long, 100 nm wide Si nanowire field effect devices, which were successfully used for label-free DNA/PNA hybridization detection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available