Journal
ACS NANO
Volume 2, Issue 5, Pages 841-846Publisher
AMER CHEMICAL SOC
DOI: 10.1021/nn700303f
Keywords
interferometry; cytoskeleton remodeling; live-cell imaging
Categories
Funding
- NCI NIH HHS [R01 CA090571, R01CA90571, R01 CA107300, R01CA107300] Funding Source: Medline
- NEI NIH HHS [PN2 EY018228-02, PN2EY018228, PN2 EY018228] Funding Source: Medline
- NIGMS NIH HHS [R01GM073981, R21GM074509, R01 GM073981, R21 GM074509] Funding Source: Medline
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Cancer and many other diseases are characterized by changes in cell morphology, motion, and mechanical rigidity. However, in live cell cytology, stimulus-induced morphologic changes typically take 10-30 min to detect. Here, we employ live-cell interferometry (LCI) to visualize the rapid response of a whole cell to mechanical stimulation, on a time scale of seconds, and we detect cytoskeletal remodeling behavior within 200 s. This behavior involved small, rapid changes in cell content and miniscule changes in shape; it would be difficult to detect with conventional or phase contrast microscopy alone and is beyond the dynamic capability of AFM. We demonstrate that LCI provides a rapid, quantitative reconstruction of the cell body with no labeling. This is an advantage over traditional microscopy and flow cytometry, which require cell surface tagging and/or destructive cell fixation for labeling
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