Journal
ACS CHEMICAL NEUROSCIENCE
Volume 4, Issue 4, Pages 575-584Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cn3002015
Keywords
beta-Amyloid; carbohydrates; cytotoxicity; magnetic glyconanoparticles; magnetic resonance imaging
Funding
- National Institutes of Health [R01CA149451-01A1]
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The development of a noninvasive method for the detection of Alzheimer's disease is of high current interest, which can be critical in early diagnosis and in guiding treatment of the disease. The aggregates of beta-amyloid are a pathological hallmark of Alzheimer's disease. Carbohydrates such as gangliosides have been shown to play significant roles in initiation of amyloid aggregation. Herein, we report a biomimetic approach using superparamagnetic iron oxide glyconanoparticles to detect beta-amyloid. The bindings of beta-amyloid by the glyconanoparticles were demonstrated through several techniques including enzyme linked immunosorbent assay, gel electrophoresis, tyrosine fluorescence assay, and transmission electron microscopy. The superparamagnetic nature of the nanoparticles allowed easy detection of beta-amyloid both in vitro and ex vivo by magnetic resonance imaging. Furthermore, the glyconanoparticles not only were nontoxic to SH-SY5Y neuroblastoma cells but also greatly reduced beta-amyloid induced cytotoxicity to cells, highlighting the potential of these nanoparticles for detection and imaging of beta-amyloid.
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