Journal
ACS CHEMICAL BIOLOGY
Volume 9, Issue 10, Pages 2359-2365Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cb500502n
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Funding
- Ministry of Science and Technology [102-2113-M007-004-MY2, 101-2113-M009-006-MY2]
- Ministry of Education, Taiwan (ROC) [102N2011E1]
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One major limitation of labeling proteins with synthetic fluorophores is the high fluorescence background, which necessitates extensive washing steps to remove unreacted fluorophores. In this paper, we describe a novel fluorogenic probe based on an environment-sensitive fluorophore for labeling with SNAP-tag proteins. The probe exhibits dramatic fluorescence turn-on of 280-fold upon being labeled to SNAP-tag. The major advantages of our fluorogenic probe are the dramatic fluorescence turn-on, ease of synthesis, high selectivity, and rapid labeling with SNAP-tag. No-wash labeling of both intracellular and cell surface proteins was successfully achieved in living cells, and the localization of these proteins was specifically visualized.
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