Journal
ACS CHEMICAL BIOLOGY
Volume 8, Issue 9, Pages 1900-1906Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cb400369u
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- French Agence Nationale de la Recherche [ANR-08-BLAN-0208-01, ANR-09-BLAN-0241]
- Agence Nationale de la Recherche (ANR) [ANR-08-BLAN-0208, ANR-09-BLAN-0241] Funding Source: Agence Nationale de la Recherche (ANR)
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While chitooligosaccharides (COs) derived from fungal chitin are potent elicitors of defense reactions, structurally related signals produced by certain bacteria and fungi, called lipo-chitooligosaccharides (LCOs), play important roles in the establishment of symbioses with plants. Understanding how plants distinguish between friend and foe through the perception of these signals is a major challenge. We report the synthesis of a range of COs and LCOs, including photoactivatable probes, to characterize a membrane protein from the legume Medicago truncatula. By coupling photoaffinity labeling experiments with proteomics and transcriptomics, we identified the likely LCO-binding protein as LYR3, a lysin motif receptor-like kinase (LysM-RLK). LYR3, expressed heterologously, exhibits high-affinity binding to LCOs but not COs. Homology modeling, based on the Arabidopsis CO-binding LysM-RLK AtCERK1, suggests that LYR3 could accommodate the LCO in a conserved binding site. The identification of LYR3 opens up ways for the molecular characterization of LCO/CO discrimination.
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