Journal
ACS CHEMICAL BIOLOGY
Volume 8, Issue 3, Pages 535-542Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cb300625g
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Funding
- Russian foundation for basic research (RFBR) [10-04-01561-a, 11-04-12187-ofi]
- EMBL-RFBR [12-04-92427]
- Measures to Attract Leading Scientists to Russian Educational Institutions program [11.G34.31.0017]
- ESF EuroMembrane consortium TraPPs
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High-performance sensors for reactive oxygen species are instrumental to monitor dynamic events in cells and organisms. Here, we present HyPer-3, a genetically encoded fluorescent indicator for intracellular H2O2 exhibiting improved performance with respect to response time and speed. HyPer-3 has an expanded dynamic range compared to HyPer and significantly faster oxidation/reduction dynamics compared to HyPer-2. We demonstrate this performance by in vivo imaging of tissue scale H2O2 gradients in zebrafish larvae. Moreover, HyPer-3 was successfully employed for single wavelength fluorescent lifetime imaging of H2O2 levels both in vitro and in vivo.
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