Journal
ACS CHEMICAL BIOLOGY
Volume 6, Issue 9, Pages 893-899Publisher
AMER CHEMICAL SOC
DOI: 10.1021/cb200094w
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- HHMI
- NIH
- NSF
- SMA2
- SMART
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Phenazines, a group of fluorescent small molecules produced by the bacterium Pseudomonas aeruginosa, play a role in maintaining cellular redox homeostasis. Phenazines have been challenging to study in vivo due to their redbx activity, presence both intra- and extracellularly, and their diverse chemical properties. Here, we describe a noninvasive in vivo optical technique to monitor phenazine concentrations within bacterial cells using time-lapsed spectral multiphoton fluorescence microscopy. This technique enables simultaneous monitoring of multiple weakly fluorescent molecules (phenazines, siderophores, NAD(P)H) expressed by bacteria in culture. This work provides phenazine-NAD(P)H redox system in Pseudomonas aeruginosa, illuminating an unanticipated role for 1-hydroxyphenazine. Similar approaches could be used to study the abundance and redox dynamics of the a wide range of small molecules within bacteria both us single cells and in communities.
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