Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 32, Pages 26851-26858Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b05447
Keywords
plasmon-enhanced fluorescence; gold nanobipyramids; telomerase activity; in situ imaging; turn on
Funding
- National Nature Science Foundation of China [21675093, 21505081]
- QUST [010022832]
- Taishan Scholar Program of Shandong Province, China [ts20110829]
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Herein, we developed a novel plasmon-enhanced fluorescence (PEF)-based telomerase-responsive nanoprobe for in situ fluorescence turn on visualization of telomerase activity in live cells. The as-prepared nanoprobe was composed of a nicked molecular beacon (which contains Cy5.5-labeled hairpin-DNA sequences hybridized with telomerase primers)-functionalized gold nanobipyramids (Au NBPs). Au NBPs were selected as both fluorescence resonance energy-transfer and PEF dual-functional substrates, while DNA was selected to be the precise spacer to manage the interval between the Au NBPs and Cy5.5. On the basis of this target-triggered PEF probe, optimal fluorescence enhancement can be obtained with 49 DNA bases, which was higher than gold nanorods. The proposed method accomplishes sensitive telomerase activity detection down to 23 HeLa cells with a dynamic range of 40-1200 HeLa cells. On the basis of this, in situ fluorescence imaging of telomerase activity in live cells and real-time analysis of the variation in intracellular telomerase activity can be achieved. Moreover, cancer cells and normal cells can also be successfully discriminated even in their co-cultured mixtures, indicating promising potential in clinical diagnoses.
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