Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 10, Issue 39, Pages 33416-33422Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.8b09696
Keywords
silver nanowires; thermometer; fluorescence; DNA stem-loop; single cell
Funding
- National Key R&D Program of China [2016YFA0200800]
- NSFC [51672284]
- Beijing Natural Science Foundation [2181002]
- Chinese Academy of Sciences [1A1111KYSB20180017, QYZDJ-SSW-JSC032, XDB17000000]
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A fluorescence thermometer based on silver nanowires (AgNWs) is realized by assembling Texas Red (TR)-marked thermal-sensitive DNA stem loops (TR-DNA stem loop) on the surface of AgNWs. Temperature configures the structure of the TR-DNA stem loop and resultantly adjusts the energy transfer between TR and the AgNWs, which could sensitively control the fluorescence intensity of the thermometer. The thermometer is sensitive to the temperature ranging from 30 to 40 degrees C with the sensitivity of 2.6%/degrees C. Under the assistance of laser confocal microscopy, a temperature change within a single cell was observed by the monofilament AgNW-based thermometer.
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