Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 6, Issue 13, Pages 10005-10013Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am502537k
Keywords
carboxymethyl chitosan; gentamycin sulfate; hydrogel; biofilm; membrane permeability
Funding
- National Basic Research program [2012cb619103]
- National Support Program for Science and Technology [2012BAI17B06]
- Natural Science Foundation [81271702, 31170922]
- Program for New Century Excellent Talents in University [NCET-12-0387]
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It is critical for the clinical success to take the biological function into consideration when integrating the antibacterial function into the implanted biomaterials. To this aim, we prepared gentamycin sulfate (GS)-loaded carboxymethyl-chitosan (CM-chitosan) hydrogel cross-linked by genipin. The prepared hydrogels not only achieved superb inhibition on bacteria growth and biofilm formation of Staphylococcus aureus but also significantly enhanced the adhesion, proliferation, and differentiation of MC3T3-E1 cells. The observed dual functions were likely based on the intrinsic property of the positive charged chitosan-based hydrogel, which could be modified to selectively disrupt the bacteria wall/membrane and promote cell adhesion and proliferation, as suggested by the membrane permeability study. The genipin concentration played an important role in controlling the degradation time of the chitosan hydrogel and the MC3T3-E1 cell responses. The loading of GS not only significantly increased the antibacterial efficiency but also was beneficial for the osteoblastic cell responses. Overall, the biocompatibility of the prepared chitosan-GS hydrogel could be tuned with both the genipin and GS concentrations, which control the available positive charged sites of chitosan. The results demonstrated that chitosan-GS hydrogel is an effective and simple approach to achieving combined antibacterial efficacy and excellent osteoblastic cell responses, which has great potential in orthopedic applications.
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