Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 6, Issue 15, Pages 13234-13241Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am503003v
Keywords
metal mesh device; glycopolymer; label-free biosensor; protein; bacteria
Funding
- [24655157]
- [23685027]
- Grants-in-Aid for Scientific Research [13J05206, 26620106, 23685027] Funding Source: KAKEN
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Biosensors for the detection of proteins and bacteria have been developed using glycopolymer-immobilized metal mesh devices. The trimethoxysilane-containing glycopolymer was immobilized onto a metal mesh device using the silane coupling reaction. The surface shape and transmittance properties of the original metal mesh device were maintained following the immobilization of the glycopolymer. The mannose-binding protein (concanavalin A) could be detected at concentrations in the range of 10(-9) to 10(-6) mol L-1 using the glycopolymer-immobilized metal mesh device sensor, whereas another protein (bovine serum albumin) was not detected. A detection limit of 1 ng mm(-2) was achieved for the amount of adsorbed concanavalin A. The glycopolymer-immobilized metal mesh device sensor could also detect bacteria as well as protein. The mannose-binding strain of Escherichia coli was specifically detected by the glycopolymer-immobilized metal mesh device sensor. The glycopolymer-immobilized metal mesh device could therefore be used as a label-free biosensor showing high levels of selectivity and sensitivity toward proteins and bacteria.
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