Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 6, Issue 20, Pages 17857-17864Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am505876b
Keywords
sulfhydryl cotton fiber (SCF); thiol-ene click chemistry; boronate affinity; immobilized metal ion affinity chromatography (IMAC); biological analysis
Funding
- National Basic Research Program of China (973 Program) [2013CB910702]
- National Natural Science Foundation of China [91217309, 91017013]
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Sulfhydryl cotton fiber (SCF) has been widely used as adsorbent for a variety of metal ions since 1971. Thanks to the abundant thiols on SCF, in this study, we reported a universal method for the facile preparation of SCF-based materials using thiol-ene click chemistry for the first time. With the proposed method, two types of SCF-based materials, phenylboronic acid grafted sulfhydryl cotton fiber (SCF-PBA) and zirconium phosphonate-modified sulfhydryl cotton fiber (SCF-pVPA-Zr4+), were successfully prepared. The grafted functional groups onto the thiol group of SCF were demonstrated by X-ray photoelectron spectroscopy (XPS) and energy dispersive X-ray spectroscopy (EDX). The prepared fibrous materials exhibited excellent fiber strength, good stability in aqueous or nonaqueous solutions, and great biocompatibility. Moreover, we developed filter-free in-pipet-tip SPE using these SCF-based materials as adsorbent for the enrichment of ribonucleosides, glycopeptides and phosphopeptides. Our results showed that SCF-PBA adsorbent can selectively capture ribonucleosides and glycopeptides from complex biological samples. And SCF-pVPA-Zr4+ adsorbent exhibited high selectivity and capacity in the enrichment of phosphopeptides from the digestion mixture of beta-casein and bovine serum albumin (BSA), as well as human serum and nonfat milk digest. Generally, the preparation strategy can be a universal method for the synthesis of other functionalized cotton-based adsorbents with special requirement in microscale biological analysis.
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