Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 6, Issue 8, Pages 5900-5907Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am5008134
Keywords
localized gene delivery; nano-graphene oxide; patterned substrates; nano-bio intetface
Funding
- Major State Basic Research Development Program [2013CB932702, 2012CB932601]
- National Natural Science Foundation of China [21275106]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
- Youth 1000-plan in the Recruitment Program of Global Experts
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A facile approach was developed to fabricate patterned substrates of nano-graphene oxide, demonstrating highly localized and efficient gene delivery to multiple cell lines in a substrate-mediated manner. The GO substrates served as a valid platform to preconcentrate PEI/pDNA complexes and maintain their gradual releasing for a relatively long period of time. Our approach allowed successful gene delivery in selected groups of cells on the stripe-patterned GO substrates, without transfecting their neighbor cells directly cultured on glass. These GO substrates exhibited excellent biocompatibility and enabled effective gene transfection for various cell lines including stem cells, thus promising important applications in stem cell research and tissue engineering.
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