Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 6, Issue 15, Pages 12752-12760Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am5027067
Keywords
gigaporous; coating; polystyrene particles; cross-linked poly(vinyl alcohol) hydrogel; protein adsorption
Funding
- National Natural Science Foundation of China [21176257, 21276280]
- Natural Science Foundation for Distinguished Young Scholar of Shandong Province [JQ 201008]
- key Science and Technology Program of Qingdao Economic & Technical Development Zone
- Fundamental Research Funds for the Central Universities
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Gigaporous polystyrene (PS) microspheres were hydrophilized by in situ polymerization to give a stable cross-linked poly(vinyl alcohol) (PVA) hydrogel coating, which can shield proteins from the hydrophobic PS surface underneath. The amination of microspheres (PS-NH2) was first carried out through acetylization, oximation and reduction, and then 4,4'-azobis (4-cyanovaleric acid) (ACV), a polymerization initiator, was covalently immobilized on PS-NH2 through amide bond formation, and the cross-linked poly(vinyl acetate) (PVAc) was prepared by radical polymerization at the surfaces of ACV-immobilized PS microspheres (PS-ACV). Finally, the cross-linked PVA hydrogel coated gigaporous PS microspheres (PS-PVA) was easily achieved through alcoholysis of PVAc. Results suggested that the PS microspheres were effectively coated with cross-linked PVA hydrogel, where the gigaporrous structure remained under optimal conditions. After hydrophilic modification (PS-PVA), the protein-resistant ability of microspheres was greatly improved. The hydroxyl-rich PS-PVA surface can be easily derivatized by classical chemical methods. Performance advantages of the PS-PVA column in flow experiment include good permeability, low backpressure, and mechanical stability. These results indicated that PS-PVA should be promising in rapid protein chromatography.
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