Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 6, Issue 20, Pages 17543-17550Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am505501d
Keywords
fluorescent probe; ratiometric; cysteine; ESIPT; selectivity; bioimaging
Funding
- National Natural Science Foundation of China [21172086, 21032001, 21272084]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry
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We report a simple and readily available fluorescent probe for rapid, specific, and ratiometric fluorescent detection of the biologically important cysteine (Cys). This probe uses a visible-light excitable excited-state intramolecular proton transfer (ESIPT) dye (4'-dimethylamino-3-hydroxyflavone) as the fluorophore and an acrylate group as the ESIPT blocking agent as well as the recognition unit. Cleavage of the acrylate moiety can be achieved specifically and rapidly by Cys in aqueous solution under mild conditions, which leads to restore the ESIPT process and enables the probe to show a rapid, ratiometric fluorescent detection process for Cys with high selectivity over various analytes, including homocysteine (Hcy) and glutathione (GSH). The detection limit of this probe for Cys was found to be similar to 0.2 mu M and bioimaging of intracellular Cys by this probe was successfully applied in living cells, indicating that this probe holds great potential for biological applications.
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