Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 5, Issue 20, Pages 9991-9997Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am403523d
Keywords
polydopamine microcapsules; enzyme immobilization; hierarchical wall structure; template-mediated method
Funding
- National Basic Research Program of China [2009CB724705]
- National Science Fund for Distinguished Young Scholars [21125627]
- Program of Introducing Talents of Discipline to Universities [B06006]
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Microcapsules with diverse wall structures may exhibit different performance in specific applications. In the present study, three kinds of mussel-inspired polydopamine (PDA) microcapsules with different wall structures have been prepared by a template-mediated method. More specifically, three types of CaCO3 microspheres (poly(allylamine hydrochloride), (PAH)-doped CaCO3; pure-CaCO3; and poly(styrene sulfonate sodium), (PSS)-doped CaCO3) were synthesized as sacrificial templates, which were then treated by dopamine to obtain the corresponding PDA-CaCO3 microspheres. Through treating these microspheres with disodium ethylene diamine tetraacetic acid (EDTA-2Na) to remove CaCO3, three types of PDA microcapsules were acquired: that was (1) PAH-PDA microcapsule with a thick (similar to 600 nm) and highly porous capsule wall composed of interconnected networks, (2) pure-PDA microcapsule with a thick (similar to 600 nm) and less porous capsule wall, (3) PSS-PDA microcapsule with a thin (similar to 70 nm) and dense capsule wall. Several characterizations confirmed that a higher degree in porosity and interconnectivity of the capsule wall would lead to a higher mass transfer coefficient. When serving as the carrier for catalase (CAT) immobilization, these enzyme-encapsulated PDA microcapsules showed distinct structure-related activity and stability. In particular, PAH-PDA microcapsules with a wall of highly interconnected networks displayed several significant advantages, including increases in enzyme encapsulation efficiency and enzyme activity/stability and a decrease in enzyme leaching in comparison with other two types of PDA microcapsules. Besides, this hierarchically structured PAH-PDA microcapsule may find other promising applications in biocatalysis, biosensors, drug delivery, etc.
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