Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 5, Issue 11, Pages 4549-4554Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am4001985
Keywords
conjugated polymers; DNA alkylation; plasmid; GFP; gene regulation; protein expression
Funding
- National Natural Science Foundation of China [21033010, 21021091, 21203213, 21273254]
- Major Research Plan of China [2011CB808400, 2013CB932800]
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A new polyfluorene derivative containing pendent alkylating chlorambucil (PFP-Cbl) was synthesized and characterized. Under direct incubation with DNA in vitro, PFP-Cbl could undergo an efficient DNA alkylating reaction and induce DNA cross-linking. In vitro transcription and translation experiment exhibited that the PFP-Cbl significantly down-regulated the gene expression of luciferase reporter plasmid. The down-regulation of gene expression was also verified through the transfection experiment of p-EGFP plasmid, which showed decreased green fluorescent protein (GFP) in cells. Meanwhile, the self-luminous property of PFP-Cbl could make it able to trace the internalized, PFP-Cbl and plasmid complexes resulted from cross-linking in cells by fluorescent microscopy. Combining the features of alkylating function, multivalent binding sites, and fluorescent characteristics, PFP-Cbl provides a new insight in the area of gene regulation and extends the new applications of conjugated polymers (CPs).
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