Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 5, Issue 19, Pages 9798-9802Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am4029735
Keywords
caspase-3; inner-filter effect; gold nanoparticle; quantum dot; fluorescence
Funding
- National Science Fund for Distinguished Young Scholars [201125418]
- National Science Foundation of China [21175147]
- Project of Scientific and Technologic Infrastructure of Suzhou [SZS201207]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
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We describe here a simple fluorometric assay for the highly sensitive detection of caspase-3 activities on the basis of the inner-filter effect of gold nanoparticles (AuNPs) on CdTe quantum dots (QDs). The method takes advantage of the high molar absorptivity of the plasmon band of gold nanoparticles as well as the large absorption band shift from 520 to 680 nm upon nanoparticle aggregation. When labeled with a peptide possessing the caspase-3 cleavage sequence (DEVD), the monodispersed Au-Ps (peptide-modified AuNPs) exhibited a tendency to aggregate when exposed to caspase-3, which induced the absorption band transition from 520 to 680 nm and turned on the fluorescence of the CdTe QDs for caspase3 sensing. Under optimum conditions, a high sensitivity towards caspase-3 was achieved with a detection limit as low as 18 pM, which was much lower than the corresponding assays based on absorbance or other approaches. Overall, we demonstrated a facile and sensitive approach for caspase-3 detection, and we expected that this method could be potentially generalized to design more fluorescent assays for sensing other bioactive entities.
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