Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 5, Issue 17, Pages 8430-8439Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am4017945
Keywords
biodegradable; fibrin; hydrogel; inducible; nitric oxide; tissue engineering
Funding
- National Science Foundation, Division of Materials Research [DMR-0906709-2009]
Ask authors/readers for more resources
An S-nitroso-N-acetylpenicillarnine (SNAP) derivatization approach was used to modify existing free primary amines found in fibrin (a natural protein-based biomaterial) to generate a controlled nitric oxide (NO) releasing scaffold material. The duration of the derivatization reaction affects the NO release kinetics, the induction of controlled NO-release, hydrophobicity, swelling behavior, elastic moduli, rheometric character, and degradation behavior. These properties were quantified to determine changes in fibrin hydrogels following covalent attachment of SNAP. NO-releasing materials exhibited minimal cytotoxicity when cultured with fibroblasts or osteoblasts. Cells maintained viability and proliferative character on derivatized materials as demonstrated by Live/Dead cell staining and counting. In addition, SNAP-derivatized hydrogels exhibited an antimicrobial character indicative of NO-releasing materials. SNAP derivatization of natural polymeric biomaterials containing free primary amines offers a means to generate inducible NO-releasing biomaterials for use as an antimicrobial and regenerative support for tissue engineering.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available