Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 4, Issue 12, Pages 6450-6453Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am302268t
Keywords
graphene oxide; hybridization chain reaction; MicroRNA; fluorescence sensor; enzyme-free; fluorescence imaging
Funding
- National Natural Science Foundation of China [20925519, 91127035, 21105020]
- Natural Science Foundation of Hebei Province [B2011201175, B2012201108]
Ask authors/readers for more resources
A new enzyme-free signal amplification-based assay for microRNA (miRNA) detection is developed by using hybridization chain reaction (HCR) coupled with a graphene oxide (GO) surface-anchored fluorescence signal readout pathway. MiRNAs can efficiently initiate HCR between two species of fluorescent hairpin probes. After HCR, both of the excess hairpin probes and the HCR products will be anchored on the GO surface. The fluorescence of the hairpin probes can be completely quenched by GO, whereas the HCR products maintain strong fluorescence. Therefore, integrating HCR strategy for signal amplification with selective fluorescence quenching effects of GO provides a versatile miRNA assay.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available