Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 4, Issue 2, Pages 826-831Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am201483h
Keywords
atom transfer radical polymerization (ATRP); DNA sensors; fluorescence; cationic polymers; oligonucleotides; biomarkers
Funding
- Health Research Board (HRB) of Ireland
- Science Foundation Ireland (SFI)
- DEBRA Ireland
- National University of Ireland, Galway
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Cationic polymers with various structures have been widely investigated in the areas of medical diagnostics and molecular, biology because of their unique binding properties and capability to interact with biological molecules in complex biological environments.; In this work-we report the grafting of a linear cationic polymer from an atom transfer radical polymerization (ATRP) initiator bound to cellulose paper surface. We show successful binding of ATRP initiator onto cellulose paper and grafting of polymer chains from the immobilized initiator with ATRP. The cellulose paper grafted polymer was used in combination with PicoGreen (PG) to demonstrate detection of nucleic acids in the nanogram range in homogeneous solution and in a biological sample (serum). The results showed specific identification of hybridized DNA after addition of PG in both solutions.
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