Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 3, Issue 9, Pages 3285-3292Publisher
AMER CHEMICAL SOC
DOI: 10.1021/am200604q
Keywords
XPS; Si 2p; silanes; mixed monolayer; DNA immobilization; DNA hybridization
Funding
- National Renewable Energy Laboratory (NREL) Laboratory Directed Research and Development as part of DOE [DE-AC36-99GO10337]
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The amine density of 3-aminopropyldimethylethoxysilane (APDMES) films on silica is controlled to determine its effect on DNA probe density and subsequent DNA hybridization. The amine density is tailored by controlling the surface reaction time of (1) APDMES, or (2) n-propyldimethylchlorosilane (PDMCS, which is not amine terminated) and then reacting it with APDMES to form a mixed monolayer. High-resolution X-ray photoelectron spectroscopy (XPS) is used to quantify silane surface coverage of both pure and mixed monolayers on silica; the XPS data demonstrate control of amine density in both pure APDMES and PDMCS/APDMES mixed monolayers. A linear correlation between the atomic concentration of N atoms from the amine and Si atoms from the APDMES in pure APDMES films allows us to calculate the PDMCS/APDMES ratio in the mixed monolayers. Fluorescence from attached DNA probes and from hybridized DNA decreases as the percentage of APDMES in the mixed monolayer is decreased by dilution with PDMCS.
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