Journal
JOURNAL OF BIOMEDICAL SCIENCE
Volume 18, Issue -, Pages -Publisher
BIOMED CENTRAL LTD
DOI: 10.1186/1423-0127-18-39
Keywords
STC-1; angiogenesis; VEGF; PKC beta II; ERK1/2
Categories
Funding
- National Natural Science Foundation of China [30872941]
- Fundamental Research Funds for the Central Universities [1106020822]
- Nanjing Medical Science and Technique Development Foundation (Personalized Therapy of Non-small Cell Lung Cancer Patients), the Scientific Research Foundation of Graduate School of Nanjing University [2008CL06]
Ask authors/readers for more resources
Background: Stanniocalcin-1(STC-1) is up-regulated in several cancers including gastric cancer. Evidences suggest that STC-1 is associated with carcinogenesis and angiogenic process. However, it is unclear on the exact role for STC-1 in inducing angiogenesis and tumorigeneisis. Method: BGC/STC cells (high-expression of STC-1) and BGC/shSTC cells (low-expression of STC-1) were constructed to investigate the effect of STC-1 on the xenograft tumor growth and angiogenesis in vitro and in vivo. ELISA assay was used to detect the expression of vascular endothelial growth factor (VEGF) in the supernatants. Neutralizing antibody was used to inhibit VEGF expression in supernatants. The expression of phosphorylated -PKCbII, phosphorylated -ERK1/2 and phosphorylated -P38 in the BGC treated with STC-1protein was detected by western blot. Results: STC-1 could promote angiogenesis in vitro and in vivo, and the angiogenesis was consistent with VEGF expression in vitro. Inhibition of VEGF expression in supernatants with neutralizing antibody markedly abolished angiogenesis induced by STC-1 in vitro. The process of STC-1-regulated VEGF expression was mediated via PKCbII and ERK1/2. Conclusions: STC-1 promotes the expression of VEGF depended on the activation of PKCbII and ERK1/2 pathways. VEGF subsequently enhances tumor angiogenesis which in turn promotes the gastric tumor growth.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available