Journal
MASS SPECTROMETRY LETTERS
Volume 2, Issue 3, Pages 61-64Publisher
KOREAN SOC MASS SPECTROMETRY
DOI: 10.5478/MSL.2011.2.3.061
Keywords
MALDI-TOF-MS; Glycan analysis; Prespotted MALDI plate
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Funding
- Ministry of Knowledge Economy (MKE), Korea [10035490]
- Korea Evaluation Institute of Industrial Technology (KEIT) [10035490] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Matrix Assisted Laser Desorption/Ionization-Time-of-Flight mass spectrometry (MALDI-TOF-MS) is the most widely used MS technique for glycan analysis. However, the poor point-to-point and sample-to-sample reproducibility becomes a limit in glycan biomarker research. A prespotted MALDI plate which overcomes the large crystal formation of 2,5-dihydroxybenzoic acid (DHB) has been developed and applied for glycan analysis. A homogeneous matrix coated surface without a crystal structure was formed on a hydrophilic/hydrophobic patterned surface using a piezoelectric device. The reproducible MALDI-TOF-MS data have been presented using MALDI imaging of beer glycan as well as serum glycan eluted from 10% and 20% ACN elution fractions. The glycan profile from the serum glycan by MALDI-TOF-MS with a DHB prespotted plate was highly conserved for 10 different spectra and the coefficient of variations of significant ion peaks of MALDI data varies from 3.59 to 19.95.
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