Journal
JOURNAL OF THE KOREAN SURGICAL SOCIETY
Volume 81, Issue 3, Pages 176-186Publisher
KOREAN SURGICAL SOCIETY
DOI: 10.4174/jkss.2011.81.3.176
Keywords
Adipose tissue-derived stem cell; Mesenchymal stem cells; Acute liver injury; Stem cell transplantation
Categories
Funding
- Astellas Pharma Korea Inc. [5-2006-D0237-00004]
- Gangneung Dong-In Hospital
- Clinical Research Institute, Daejeon St. Mary's Hospital
- Catholic University of Korea School of Medicine [CMCDJ-P-2011-001]
Ask authors/readers for more resources
Purpose: Even though adipose tissue-derived stem cells (ADSCs) have been spotlighted as a possible alternative for liver transplantation in an experimental setting, the mechanism by which ADSCs improve liver dysfunction remains poorly characterized. The objective of this study was to evaluate the therapeutic ability of undifferentiated ADSCs, and find a few clues on how ADSCs alleviate liver damage by comparing the transplantation routes. Methods: In vitro generated human ADSCs were checked for surface markers and stage-specific genes for characterization. Afterwards, they were transplanted into C57BL/6 mice with CCl4-induced liver injury. The transplantations were made via tail vein, portal vein, and direct liver parenchymal injection. At 1 and 3 post-transplantation days, serum biochemical parameters and/or liver specimens were evaluated. Results: We have shown here that ADSCs have the characteristics of mesenchymal stem cells, and belong to endo-dermal and/or early hepatic differentiation stage. After transplantation into the mice with acute liver failure, markers of liver injury, such as alanineaminotransferase, aspartateaminotransferase, as well as ammonia, decreased. Of these transplantation routes, transplantation via tail vein rendered the most prominent reduction in the biochemical parameters. Conclusion: Undifferentiated ADSCs have the ability to improve hepatic function in mice with acute liver injury. Moreover, our transplantation route study supports the theory that ADSCs in systemic circulation can exert endocrine or paracrine effects to ameliorate the injured liver.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available