Journal
NATURE BIOMEDICAL ENGINEERING
Volume 2, Issue 3, Pages 173-182Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41551-018-0200-7
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Funding
- Start-Up Grant for Young Scientists (Kyoto University)
- iCeMS Accelerated Project Grant (iCeMS, Kyoto University)
- JSPS (Japan Society for Promotion Science) [24680052, 15H03022]
- New Energy and Industrial Technology Development Organization (NEDO) Japan [P10027, P14006]
- Japan Agency for Medical Research and Development (AMED)
- iCeMS Overseas Visit Program for Young Researchers
- Grants-in-Aid for Scientific Research [15H03022, 24680052] Funding Source: KAKEN
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The large-scale and cost-effective production of quality-controlled human pluripotent stem cells (hPSCs) for use in cell therapy and drug discovery would ideally require a chemically defined xenobiotic-free culture system. Towards the development of such a system, costs associated with the use of recombinant proteins as supplements in basal culture media need to be reduced. Here, we describe a growth-factor-free culture medium that uses just three chemical compounds and a lower number of recombinant proteins than used in commercially available media. We show that the culture medium supports the long-term propagation of hPSCs, as confirmed by karyotype, the expression of pluripotency markers and the capacity to differentiate into cell types derived from the three embryonic germ layers. hPSCs growing in the medium were less dependent on glycolytic pathways than cells grown in medium containing growth factors. Moreover, the medium supported the generation of induced pluripotent stem cells derived from either human dermal fibroblasts or peripheral blood mononuclear cells. Our findings should facilitate the ongoing development of a completely xeno-free, chemically defined, synthetic culture system for hPSCs.
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