Journal
MITOCHONDRIAL DNA PART B-RESOURCES
Volume 3, Issue 1, Pages 375-377Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/23802359.2018.1450686
Keywords
Illumina sequencing; mitogenomics; inquiry-based learning; Trichoptera; Phryganeidae
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Funding
- University of Manitoba Research Grants Program
- Faculty of Science Field Work Support Program
- CATL Teaching and Learning Enhancement Fund
- Natural Sciences and Engineering Research Council (NSERC) of Canada [RGPIN386337-2011, RGPIN-2016-06012]
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The rush sedge caddisfly Phryganea cinerea Walker, 1852 (Phryganeidae, the giant casemakers), is a widespread and adaptable North American caddisfly. Genome skimming by Illumina sequencing permitted the assembly of a complete 15,043 bp circular mitogenome from P. cinerea consisting of 78.2% AT nucleotides, 22 tRNAs, 13 protein-coding genes, 2 rRNAs and a control region in the ancestral insect gene order. Phryganea cinerea COX1 features an atypical CGA start codon and COX1, NAD1, NAD4, and NAD5 exhibit incomplete stop codons completed by the addition of 3' A residues to the mRNA. Phylogenetic reconstruction reveals a monophyletic Order Trichoptera and Family Phyrganeidae.
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