Journal
ZOOLOGICAL LETTERS
Volume 4, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s40851-018-0088-9
Keywords
Above-and below-ground linkage; Collembola (springtails); DNA barcoding; Ecological communities; Food webs; Illumina sequencing; Insects; Networks; Molecular gut content analyses; Predator-prey interactions
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Funding
- JSPS KAKENHI Grant [26650144]
- JST PRESTO [JPMJPR16Q6]
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Background: Understanding feedback between above-and below-ground processes of biological communities is a key to the effective management of natural and agricultural ecosystems. However, as above-and below-ground food webs are often studied separately, our knowledge of material flow and community dynamics in terrestrial ecosystems remains limited. Results: We developed a high-throughput sequencing method for examining how spiders link above-and below-ground food webs as generalist predators. To overcome problems related to DNA-barcoding-based analyses of arthropod-arthropod interactions, we designed spider-specific blocking primers and Hexapoda-specific primers for the selective PCR amplification of Hexapoda prey sequences from spider samples. By applying the new DNA metabarcoding framework to spider samples collected in a temperate secondary forest in Japan, we explored the structure of a food web involving 15 spider species and various taxonomic groups of Hexapoda prey. These results support the hypothesis that multiple spider species in a community can prey on both above-and below-ground prey species, potentially coupling above-and below-ground food-web dynamics. Conclusions: The PCR primers and metabarcoding pipeline described in this study are expected to accelerate nuclear marker-based analyses of food webs, illuminating poorly understood trophic interactions in ecosystems.
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