Journal
GENOMICS PROTEOMICS & BIOINFORMATICS
Volume 16, Issue 1, Pages 73-84Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.gpb.2017.10.002
Keywords
Autoantibody tumor biomarker; Cancer immunology; Colorectal cancer; Immunomics; Protein microarray
Categories
Funding
- Life Science Krems Fund [30]
- Jubilaumsfonds of the Austrian National Bank [15192]
- Vienna Science and Technology Fund of Austria [LS11-026]
Ask authors/readers for more resources
Characterization of the colon cancer immunome and its autoantibody signature from differentially-reactive antigens (DIRAGs) could provide insights into aberrant cellular mechanisms or enriched networks associated with diseases. The purpose of this study was to characterize the antibody profile of plasma samples from 32 colorectal cancer (CRC) patients and 32 controls using proteins isolated from 15,417 human cDNA expression clones on microarrays. 671 unique DIRAGs were identified and 632 were more highly reactive in CRC samples. Bioinformatics analyses reveal that compared to control samples, the immunoproteomic IgG profiling of CRC samples is mainly associated with cell death, survival, and proliferation pathways, especially proteins involved in EIF2 and mTOR signaling. Ribosomal proteins (e.g., RPL7, RPL22, and RPL27A) and CRC-related genes such as APC, AXIN1, E2F4, MSH2, PMS2, and TP53 were highly enriched. In addition, differential pathways were observed between the CRC and control samples. Furthermore, 103 DIR-AGs were reported in the SEREX antigen database, demonstrating our ability to identify known and new reactive antigens. We also found an overlap of 7 antigens with 48 CRC genes. These data indicate that immunomics profiling on protein microarrays is able to reveal the complexity of immune responses in cancerous diseases and faithfully reflects the underlying pathology.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available