Journal
SCIENCE ADVANCES
Volume 4, Issue 5, Pages -Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/sciadv.aat0496
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Funding
- United States Agency for International Development Combating Zika and Future Threats [AID-OAA-F-16-00100]
- Brazilian Research Councils MCTIC/FNDCT-CNPq/MEC-CAPES/MS-DecitE14/2016 [440929/2016-4]
- Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro [E18/2015]
- Government of Canada [0781-06]
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The accelerating global spread of arboviruses, such as Zika virus (ZIKV), highlights the need for more proactive mosquito surveillance. However, a major challenge during arbovirus outbreaks has been the lack of rapid and affordable tests for pathogen detection in mosquitoes. We show for the first time that near-infrared spectroscopy (NIRS) is a rapid, reagent-free, and cost-effective tool that can be used to noninvasively detect ZIKV in heads and thoraces of intact Aedes aegypti mosquitoes with prediction accuracies of 94.2 to 99.3% relative to quantitative reverse transcription polymerase chain reaction (RT-qPCR). NIRS involves simply shining a beam of light on a mosquito to collect a diagnostic spectrum. We estimated in this study that NIRS is 18 times faster and 110 times cheaper than RT-qPCR. We anticipate that NIRS will be expanded upon for identifying potential arbovirus hotspots to guide the spatial prioritization of vector control.
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