Journal
ACS SENSORS
Volume 3, Issue 4, Pages 806-814Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b00051
Keywords
MoS2; MALDI MS; chip; quantification; sulfonamides
Funding
- National Natural Science Foundation of China [21775068, 21227009, 21475060, 21405077]
- Natural Science Foundation of Jiangsu Province [BK20140591]
- National Science Fund for Creative Research Groups [21121091]
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In this work, we demonstrate, for the first time, the development of a disposable MoS2-arrayed matrix-assisted laser desorption/ionization mass spectrometry (MALDI MS) chip combined with an immunoaffinity enrichment method for high-throughput, rapid, and simultaneous quantitation of multiple sulfonamides (SAs). The disposable MALDI MS chip was designed and fabricated by MoS2 array formation on a commercial indium tin oxide (ITO) glass slide. A series of SAs were analyzed, and clear deprotonated signals were obtained in negative-ion mode. Compared with MoS2-arrayed commercial steel plate, the prepared MALDI MS chip exhibited comparable LDI efficiency, providing a good alternative and disposable substrate for MALDI MS analysis. Furthermore, internal standard (IS) was previously deposited onto the MoS2 array to simplify the experimental process for MALDI MS quantitation. 96 sample spots could be analyzed within 10 min in one single chip to perform quantitative analysis, recovery studies, and real foodstuff detection. Upon targeted extraction and enrichment by antibody conjugated magnetic beads, five SAs were quantitatively determined by the IS-first method with the linear range of 0.5-10 ng/mL (R-2 > 0.990). Good recoveries and repeatability were obtained for spiked pork, egg, and milk samples. SAs in several real foodstuffs were successfully identified and quantified. The developed method may provide a promising tool for the routine analysis of antibiotic residues in real samples.
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