Journal
ACS SENSORS
Volume 3, Issue 6, Pages 1210-1216Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b00318
Keywords
multifunctional peptide; antifouling; nonspecific adsorption; polyaniline; immunoglobulin G; biosensor
Funding
- National Natural Science Foundation of China [21422504, 21675093]
- Taishan Scholar Program of Shandong Province of China [ts20110829]
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The construction of sensitive and selective biosensors capable of detecting specific targets in complex biological samples remains a challenge highly relevant to a range of sensor/diagnostic applications. Herein, we have utilized a multifunctional peptide to present an interface that supports the very specific recruitment of targets from serum. The novel peptide sequence designed contains an anchoring domain (CPPPP-), an antifouling domain (-NQNQNQNQDHWRGWVA), and a human immunoglobulin G (IgG) recognition domain (-HWRG-WVA), and the whole peptide was designed to be antifouling. These were integrated into polyaniline nanowire arrays in supporting the quantification of IgG (with a limit of detection of 0.26 ng mL(-1)) in neat serum and real clinical samples. The strategy of utilizing multisegment peptide films to underpin highly selective target recruitment is, of course, readily extended to a broad range of targets for which an affinity sequence can be generated.
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