Journal
3 BIOTECH
Volume 8, Issue -, Pages -Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-018-1153-y
Keywords
Agrobacterium rhizogenes; Acetosyringone; Quercetin; Antioxidant activity; Radish
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Funding
- University Grant Commission: Basic Scientific Research (UGC-BSR Fellowship), Government of India, New Delhi [F.7-25/2007]
- DST-FIST Program [DST-FIST:SR/FST/LSI-618/2014]
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An efficient protocol for hairy root induction in radish was established by optimizing several parameters that affect the efficiency of Agrobacterium rhizogenes-mediated transformations. Explants wounded using sterile hypodermic needle, infected with Agrobacterium suspension (0.6 OD600) for 10 min and co-cultivated in 1/2 MS medium containing acetosyringone (100 mu M) for 2 days displayed maximum percentage of hairy root induction using MTCC 2364 (77.6%) and MTCC 532 (67.6%). On further experiments with MTCC 2364 initiated hairy roots, maximum biomass accumulation (fresh weight = 9.50 g; dry weight = 1.48 g) was achieved in liquid 1/2 MS medium supplemented with 87.6 mM sucrose after 40 days of culture. Transgenic state of hairy roots of MTCC 2364 was confirmed by polymerase chain reaction using rolB- and rolC-specific primers. The MTCC 2364-induced hairy roots produced higher amount of phenolic (33.0 mg g(-1)), flavonoid (48.0 mg g(-1)), and quercetin (114.8 mg g(-1)) content compared to auxin-induced roots of non-transformed radish. Furthermore, the results of ferric reducing antioxidant power and 1,1-diphenyl-2-picrylhydrazyl assay confirmed that the antioxidant activity of MTCC 2364 root extracts was improved when compared to auxin-induced roots of non-transformed radish. The present study offers a new insight in radish for production of phenolics and flavonoids (quercetin) using A. rhizogenes-mediated hairy root induction.
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