Journal
INFECTION AND DRUG RESISTANCE
Volume 11, Issue -, Pages 945-959Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IDR.S154255
Keywords
mycobacterium tuberculosis; MDR; molecular characterization; lncRNA; infection
Categories
Funding
- Outstanding Young scholar Foundation of Jiangsu Province, China [BK20170106]
- Collaborative Research Project of Southeast University [2242017K3DN27]
- Nanjing Medical University [2242017K3DN27]
- Medical Science and technology development Foundation, Nanjing Department of Health [ZKX13042]
- Foundation of Nanjing Medical Science and Technique Development [QRX11229]
Ask authors/readers for more resources
Purpose: The aim of this paper was to identify differentially expressed long non-coding RNAs (lncRNAs) in peripheral blood mononuclear cells (PBMCs) influenced by the infection of multidrug-resistant tuberculosis (MDR-TB). Materials and methods: IncRNA and mRNA expression profiles in PBMCs derived from healthy controls (HCs) and individuals with MDR-TB and drug-sensitive tuberculosis (DS-TB) were analyzed and compared by microarray assay. Six lncRNAs were randomly selected for validation by using real-time quantitative polymerase chain reaction (RT-qPCR). The biological functions and signaling pathways affected by the differentially expressed mRNAs were investigated by using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway-based approaches. Results: Compared with the HC group, 1,429 lncRNAs (983 mRNAs) and 2,040 lncRNAs (1,407 mRNAs) were identified to be deregulated in the MDR-TB group and in the DS-TB group, respectively, and 1,511 lncRNAs and 1,047 mRNAs were identified to be differentially expressed in both MDR-TB and DS-TB groups. Between the three groups, 22 lncRNAs and 38 mRNAs were found deregulated. Most deregulated lncRNAs were from intergenic regions (similar to 55% of the total), natural antisense to protein-coding loci (similar to 32% of the total), or intronic antisense to protein-coding loci (similar to 5% of the total). Significantly enriched signaling pathways regulated by the deregulated mRNAs were mainly associated with natural killer cell-mediated cytotoxicity, antigen processing and presentation, graft-vs-host disease, the transforming growth factor-beta signaling pathway, and the Hippo signaling pathway. Conclusion: This study is the first to report differentially expressed lncRNAs in PBMCs in response to MDR-TB infection. It revealed that some lncRNAs might be associated with regulating host immune response to MDR-TB infection. Further elucidation of the potential of these deregulated lncRNAs in MDR-TB and its reactivation requires further study.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available