Journal
INFECTION AND DRUG RESISTANCE
Volume 11, Issue -, Pages 77-86Publisher
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IDR.S145337
Keywords
antibiotic activity; polyelectrolyte network; depolymerizing factors; cathelicidin; ceragenins; DNase 1; cystic fibrosis
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Funding
- National Science Center, Poland [UMO-2015/17/B/NZ6/03473]
- NIH [P01 GM096971]
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The response of the human immune system to most bacterial infections results in accumulation of neutrophils at infection sites that release a significant quantity of DNA and F-actin. Both are negatively charged polyelectrolytes that can interact with positively charged host defense molecules such as cathelicidin-delivered LL-37 peptide or other cationic antibiotic agents. Evaluation of the ability of bacterial outgrowth (using luminescence measurements or counting colony-forming units) to form a biofilm (quantified by crystal violet staining) and analysis of the structure of DNA/F-actin network by optical microscopy in human pus samples treated with different antibiotics in combination with plasma gelsolin, DNAse 1, and/or polyaspartic acid revealed that bactericidal activity of most tested antibacterial agents increases in the presence of DNA/F-actin depolymerizing factors.
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