Journal
CELL SYSTEMS
Volume 6, Issue 3, Pages 271-+Publisher
CELL PRESS
DOI: 10.1016/j.cels.2018.03.002
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Funding
- UC Santa Cruz Genomics Institute
- NIH NHGRI [U54HG007990]
- NCI ITCR [R01CA180778]
- Oregon Health and Science University from the NCI [U24CA210957, U24CA143799]
- Keck Center of the Golf Coast Consortia for the Cancer Biology Training Program CPRIT [RP140113]
- NHGRI [U24CA211006, U01HG006517, U54HG003079, U54HG003067, U54HG003273, U24CA143843]
- NCI [U24CA143858, R01CA183793, U24CA210950, U24CA210949, U24CA143883, CA150252, U24CA143845, U24CA143835, U24CA143840, P30CA016672, U24CA143848, U24CA143882, U24CA144025, U24CA143867, U24CA143866]
- National Cancer Institute [HHSN261201400007C]
- NCI
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The Cancer Genome Atlas (TCGA) cancer genomics dataset includes over 10,000 tumor-normal exome pairs across 33 different cancer types, in total >400 TB of raw data files requiring analysis. Here we describe the Multi-Center Mutation Calling in Multiple Cancers project, our effort to generate a comprehensive encyclopedia of somatic mutation calls for the TCGA data to enable robust cross-tumor-type analyses. Our approach accounts for variance and batch effects introduced by the rapid advancement of DNA extraction, hybridization-capture, sequencing, and analysis methods over time. We present best practices for applying an ensemble of seven mutation-calling algorithms with scoring and artifact filtering. The dataset created by this analysis includes 3.5 million somatic variants and forms the basis for PanCan Atlas papers. The results have been made available to the research community along with the methods used to generate them. This project is the result of collaboration from a number of institutes and demonstrates how team science drives extremely large genomics projects.
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