4.4 Article

Isolation and identification of a vegetative organ-specific promoter from maize

Journal

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS
Volume 25, Issue 1, Pages 277-287

Publisher

SPRINGER
DOI: 10.1007/s12298-018-0546-z

Keywords

Maize; Pentatricopeptide repeat protein; Promoter; Vegetative organ specificity

Categories

Funding

  1. National Key Science and Technology Special Project [2016ZX08003004-005]
  2. National Natural Science Foundation of China [31071433]

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To avoid the unregulated overexpression of the exogenous genes, specific or inducible expression is necessary for some exogenous genes in transgenic plants. But little is known about organ- or tissue-specific promoters in maize. In the present study, the expression of a maize pentatricopeptide repeat (PPR) protein encoding gene, GRMZM2G129783, was analyzed by RNA-sequencing data and confirmed by quantitative real time PCR. The results showed that the PPRGRMZM2G129783 gene specifically expressed in vegetative organs. Consequently, a 1830bp sequence upstream of the start codon of the promoter for GRMZM2G129783 gene was isolated from maize genome (P-1830). To validate whether the promoter possesses the vegetative organ-specificity, the full-length and three 5-end deletion fragments of P-1830 of different length (1387, 437, and 146bp) were fused with glucuronidase (GUS) gene to generate promoter::GUS constructs and transformed into tobacco. The transient expression and fluorometric GUS assay in transgenic tobacco showed that all promoter could drive the expression of the GUS gene, the -437 to -146bp region possessed some crucial elements for root-specific expression, and the shortest and optimal sequence to maintain transcription activity was 146 and 437bp in length, respectively. These results indicate that the promoter of the PPRGRMZM2G129783 gene is a vegetative organ-specific promoter and will be useful in transgenic modification of commercial crops for moderate specific expression after further evaluation in monocotyledons.

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