4.4 Article

Identification of endophytic fungi from leaves of Pandanaceae based on their morphotypes and DNA sequence data from southern Thailand

Journal

MYCOKEYS
Volume -, Issue 33, Pages 25-67

Publisher

PENSOFT PUBLISHERS
DOI: 10.3897/mycokeys.33.23670

Keywords

Ascomycetes; Basidiomycota; biodiversity; phylogenetic analysis

Categories

Funding

  1. Mushroom Research Foundation (MRF), Chiang Rai, Thailand
  2. Mae Fah Luang University [592010200112]
  3. Chinese Academy of Sciences [2013T2S0030]
  4. Yunnan Provincial Department of Human Resources and Social Security [179122]
  5. National Science Foundation of China (NSFC) [31750110478, 41761144055, 41771063]

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The authors established the taxonomic status of endophytic fungi associated with leaves of Pandanaceae collected from southern Thailand. Morphotypes were initially identified based on their characteristics in culture and species level identification was done based on both morphological characteristics and phylogenetic analyses of DNA sequence data. Twenty-two isolates from healthy leaves were categorised into eight morphotypes. Appropriate universal primers were used to amplify specific gene regions and phylogenetic analyses were performed to identify these endophytes and established relationships with extant fungi. The authors identified both ascomycete and basidiomycete species, including one new genus, seven new species and nine known species. Morphological descriptions, colour plates and phylogenies are given for each taxon.

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