Journal
METHODS AND APPLICATIONS IN FLUORESCENCE
Volume 6, Issue 2, Pages -Publisher
IOP PUBLISHING LTD
DOI: 10.1088/2050-6120/aaaa05
Keywords
time dependent fluorescence shift; 6,7-dimethoxy-coumarin; hydration; water dynamics; reverse micelles
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Funding
- Czech Science Foundation [GACR 16-06096S]
- Czech Academy of Sciences
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Coumarin derivatives are well known fluorescence reporters for investigating biological systems due to their strong micro-environment sensitivity. Despite having wide range of environment sensitive fluorescence probes, the potential of 6,7-dimethoxy-coumarin has not been studied extensively so far. With a perspective of its use in protein studies, namely using the unnatural amino acid technology or as a substrate for hydrolase enzymes, we study acetyloxymethyl-6,7-dimethoxycoumarin (Ac-DMC). We investigate the photophysics and hydration dynamics of this dye in aerosol-OT (AOT) reverse micelles at various water contents using the time dependent fluorescence shift (TDFS) method. The TDFS response in AOT reverse micelles from water/surfactant ratio of 0 to 20 confirms its sensitivity towards the hydration and mobility of its microenvironment. Moreover, we show that the fluorophore can be efficiently quenched by halide ions. Hence, we conclude that the 6,7-dimethoxy-methylcoumarin fluorophore is useful for studying hydration parameters in biologically relevant systems.
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