Journal
AUSTRALASIAN PLANT DISEASE NOTES
Volume 6, Issue 1, Pages 67-71Publisher
SPRINGER
DOI: 10.1007/s13314-011-0023-9
Keywords
Actinidia chinensis; Pseudomonas syringae pv; actinidiae; Fluorescence; Te Puke; New Zealand
Categories
Funding
- Foundation for Research Science and Technology [CO2X0501]
- Low Impact Disease Control [CO6X0810]
- Zespri International
- New Zealand Institute for Plant AMP
- Food Research Limited [09-01]
- MAF
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Leaves from gold kiwifruit plants, Actinidia chinensis, with dark brown angular spots and flowers that were brown and wilted, first yielded non-fluorescent bacterial colonies following isolation. These bacterial colonies were identified by diagnostic polymerase chain reaction (PCR) as Pseudomonas syringae pv. actinidiae. These samples were obtained from the Te Puke region of New Zealand. All isolates were Gram negative and were levan positive, oxidase negative, potato soft rot negative, arginine dehydrolase negative and tobacco hypersensitivity positive (LOPAT 1a). Sequences of the gyrB and the rpoD genes of these isolates were 100% homologous to sequences of P.s. pv. actinidiae deposited in GenBank including the type strain. Koch's postulates were proven by pathogenicity tests on kiwifruit seedlings.
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