Journal
JOURNAL OF GASTROINTESTINAL ONCOLOGY
Volume 2, Issue 4, Pages 215-222Publisher
AME PUBL CO
DOI: 10.3978/j.issn.2078-6891.2011.015
Keywords
exosome; colonocyte; fecal miRNA test; miRNA; cancer screening
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Funding
- Grants-in-Aid for Scientific Research [21790547] Funding Source: KAKEN
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Background: Because the stability of miRNA in feces has not been clarified, we examined the stability of miRNA in feces. Methods: RNase was added into culture media of HT-29 cells and fecal homogenates. The relative quantifications of miRNA were analyzed by real-time RT-PCR. Results: Cellular miRNA or exosomal miRNA were protected from RNase by the cellular membrane or the exosome; meanwhile, free miRNA was degraded immediately and completely by RNase. Conclusion: The present study revealed that exosome or cellular membrane could prevent RNase from degrading miRNA inside the exosome or cells even in a dreadful condition, as in feces.
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