Journal
JOURNAL OF ORAL MICROBIOLOGY
Volume 10, Issue 1, Pages -Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/20002297.2018.1495976
Keywords
Metatranscriptome; microbiota; dental plaque; caries; children
Categories
Funding
- NIH/National Institute of Dental and Craniofacial Research at the National Institutes of Health (USA) [R37DE016937]
- William Bingham II Trust [BINGHM50]
Ask authors/readers for more resources
Background: Dental caries results from a dysbiosis of tooth-associated biofilms and frequently extends through enamel into dentin which has a different structure and composition. Objective: To evaluate the metatranscriptome of caries to determine the metabolic potential of caries communities compared with health. Design: Samples from children, caries-free (CF: n = 4) or with coronal (CC: n = 5) or dentin (DC: n = 5) caries were examined for gene expression potential. Functional profiling was performed using HUMAnN2 (HMP Unified Metabolic Analysis Network). Results: There was increased gene expression diversity in DC compared with CC and CF. Genes in CF included alcohol dehydrogenase from Neisseria sicca, methylenetetrahydrofolate reductase from Streptococcus sanguinis and choline kinase from streptococci. Genes in CC mapped mainly to Streptococcus mutans. Arginine deiminase in DC mapped to S. sanguinis and Actinomyces naeslundii. Glycerol kinase genes mapped to S. sanguinis in all groups whereas glycerol kinase in DC were from Rothia, Prevotella and streptococci. Uracil-DNA glycosylase in DC mapped to Prevotella denticola and Actinomyces. Repressor LexA in DC mapped to Scardovia wiggsiae, Dialister invisus and Veillonella parvula. Conclusions: Functional profiling revealed enzyme activities in both caries and caries-free communities and clarified marked differences between coronal and dentin caries in bacterial composition and potential gene expression.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available