Journal
JOURNAL OF ORAL MICROBIOLOGY
Volume 10, Issue -, Pages -Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/20002297.2018.1429788
Keywords
Quorum sensing; quorum quenching; AI-2; biofilms; dental plaque; streptococci
Categories
Funding
- Fundacion Ramon Areces [CIVP16A1814]
- 'Axudas do Programa de Consolidacion e Estructuracion de Unidades de Investigacion Competitivas (GPC)' from the Conselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia [ED431B2017/53]
- Conselleria de Cultura, Educacion e Ordenacion Universitaria, Xunta de Galicia [ED481A-2015/311]
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Background: Previous studies have suggested the quorum sensing signal AI-2 as a potential target to prevent the biofilm formation by Streptococcus mutans, a pathogen involved in tooth decay. Objective: To obtain inhibition of biofilm formation by S. mutans by extracts obtained from the marine bacterium Tenacibaculum sp. 20J interfering with the AI-2 quorum sensing system. Design: The AI-2 inhibitory activity was tested with the biosensors Vibrio harveyi BB170 and JMH597. S. mutans ATCC25175 biofilm formation was monitored using impedance real-time measurements with the xCELLigence system (R), confocal laser microscopy, and the crystal violet quantification method. Results: The addition of the cell extract from Tenacibaculum sp. 20J reduced biofilm formation in S. mutans ATCC25175 by 40-50% compared to the control without significantly affecting growth. A decrease of almost 40% was also observed in S. oralis DSM20627 and S. dentisani 7747 biofilms. Conclusions: The ability of Tenacibaculum sp. 20J to interfere with AI-2 and inhibit biofilm formation in S. mutans was demonstrated. The results indicate that the inhibition of quorum sensing processes may constitute a suitable strategy for inhibiting dental plaque formation, although additional experiments using mixed biofilm models would be required.
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