Journal
JOURNAL OF APPLIED CRYSTALLOGRAPHY
Volume 51, Issue -, Pages 157-166Publisher
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S1600576717018441
Keywords
lipid nanodiscs; solution scattering; small-angle X-ray scattering; wide-angle X-ray scattering; SAXS/WAXS; modelling
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Funding
- US Department of Energy, Office of Biological and Environmental Research, as part of the BER Genomic Science Program
- 'Environment Sensing and Response' Scientific Focus Area Program at Argonne National Laboratory [KP1601010]
- Life Science Biomedical Technology Research resource
- National Institute of General Medical Sciences (NIGMS) [P41 GM111244]
- DOE Office of Biological and Environmental Research [KP1605010]
- US Department of Energy, Office of Basic Energy Sciences [DE-AC02-10886]
- National Institutes of Health [S10 OD012331]
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The structural information contained in solution scattering data from empty lipid nanodiscs is examined in the context of a multi-component geometric model. X-ray scattering data were collected on nanodiscs of different compositions at scattering vector magnitudes up to 2.0 angstrom(-1). Through the calculation of the partial form factor for each of the nanodisc components before the isotropic average, structural parameters in the model were correlated to the features observed in the X-ray scattering data and to the corresponding distance distribution function. It is shown that, in general, the features at similar to 0.3-0.6 angstrom(-1) in the scattering data correlate to the bilayer structure. The data also support the argument that the elliptical shape of nanodiscs found in model fitting is physical, rather than an artefact due to the nanodisc size distribution. The lipid chain packing peak at similar to 1.5 angstrom(-1) is visible in the data and reflects the lipid bilayer phase transition. The shape change in the distance distribution function across the phase transition suggests that the nanodiscs are more circular in the fluid phase. The implication of these findings for model fitting of empty and protein-loaded nanodiscs is discussed.
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