Journal
FRONTIERS IN IMMUNOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2018.00727
Keywords
autologous dendritic cell/tumor antigen; glioblastoma multiforme; tumor-infiltrating lymphocytes; immune checkpoints; peripheral blood mononuclear cell; programmed death protein 1 (PD-1(+)); cytotoxic T-lymphocytes (CD8(+)); PD-1(+)/CD8(+) ratio
Categories
Funding
- Ministry of Science and Technology [MOST 104-2314-B-039-028, MOST 105-2314-B-039-007, MOST 106-2320-B-039-040-MY3, MOST 106-2314-B-039-019-MY3]
- Health and Welfare Surcharge of Tobacco Products, China Medical University Hospital Cancer Research Center of Excellence [MOHW107-TDU-B-212-114024]
- China Medical University and Hospital [DMR-105-155, DMR-104-047, DMR-CELL-17011, DMR-106-128]
- Research Center for New Drug Development of China Medical University
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Background: Glioblastoma (GBM) is the most common and lethal primary malignant glioma in adults. Dendritic cell (DC) vaccines have demonstrated promising results in GBM clinical trials. However, some patients do not respond well to DC therapy, with survival rates similar to those of conventional therapy. We retrospectively analyzed clinical and laboratory data to evaluate the factors affecting vaccine treatment. Methods: Forty-seven patients with de novo GBM were enrolled at China Medical University Hospital between 2005 and 2010 and divided into two subgroups. One subgroup of 27 patients received postsurgical adjuvant immunotherapy with autologous dendritic cell/tumor antigen vaccine (ADCTA) in conjunction with conventional treatment of concomitant chemoradiotherapy (CCRT) with temozolomide. The other 20 patients received only postsurgical conventional treatment without immunotherapy. Immunohistochemistry for CD45, CD4, CD8, programed death ligand 1 (PD-L1), and programed death 1 (PD-1) was performed on sections of surgical tumor specimens and peripheral blood mononuclear cells (PBMCs). Pearson's correlation, Cox proportional hazard model, and Kaplan-Meier analyses were performed to examine the correlations between the prognostic factors and survival rates. Results: Younger age (<57 years), gross total resection, and CCRT and PD-1(+) lymphocyte counts were significant prognostic factors of overall survival (OS) and progression-free survival (PFS) in the ADCTA group. Sex, CD45(+) lymphocyte count, CD4(+) or CD8(+) lymphocyte count, tumor PD-L1 expression, isocitrate dehydrogenase 1 mutation, and O6 methylguanine-DNA methyltransferase promoter methylation status were not significant factors in both groups. In the ADCTA group, patients with tumorinfiltrating lymphocytes (TILs) with a lower PD-1(+)/CD8(+) ratio (<= 0.21) had longer OS and PFS (median OS 60.97 months, P < 0.001 and PFS 11.2 months, P < 0.008) compared to those with higher PD-1(+)/CD8(+) ratio (>0.21) ( median OS 20.07 months, P < 0.001 and PFS 4.43 months, P < 0.008). Similar results were observed in patients' PBMCs; lymphocyte counts with lower PD-1(+)/CD8(+) ratio (<= 0.197) had longer OS and PFS. There was a significant correlation of PD-1(+)/CD8(+) ratio between TILs and PBMCs (Pearson's correlation R-2 = 0.6002, P < 0.001). By contrast, CD4-, CD8-, but PD-1(+), CD45(+) tumor-infiltrating lymphocytes have no impact on OS and PFS (P = 0.073 and P = 0.249, respectively). Conclusion: For patients receiving DC vaccine adjuvant therapy, better outcomes are predicted in patients with younger age, with TILs or PBMCs with lower PD-1(+)/CD8(+) ratio, with gross tumor resection, and receiving CCRT.
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