Journal
FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING
Volume 12, Issue 2, Pages 311-314Publisher
SPRINGER
DOI: 10.1007/s11705-017-1697-0
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Funding
- EPSRC
- University of Bath
- Royal Society
- Maynooth University
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A simple nitrobenzyl-umbelliferone (NCOU1) was synthesised containing a nitroreductase (NTR) trigger moiety. The presence of NTR, resulted in the fragmentation of the parent molecule and release of the highly emissive fluorophore umbelliferone via an NTR-catalyzed reduction of the nitro group. In the presence of the NTR enzyme, NCOU1 gave rise to a 5-fold increase in fluorescence intensity at 455 nm and was selective for NTR over other reductive enzymes. These results indicate that NCOU1 can be used as a simple assay for the detection of NTR.
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